The CELLFLOAT series, which combines three-dimensional rotational suspension culture equipment with cell fragmentation equipment, enabled highly efficient differentiation of human iPS cells into neural crest cells, with CD271 positivity reaching up to 92.2%. Researchers at Osaka University were involved in the experiments, and the results and application note have been published.
Differentiation of human iPS cells into neural crest cells was induced by alternately using CellPet 3D-iPS, a rotational suspension culture system, and CellPet FT, a cell fragmentation system. This method cultures cell aggregates while keeping their size uniform, with the aim of stabilizing differentiation efficiency.
Uniformly sized cell aggregates were successfully formed, with their distribution centered at approximately 300 μm in diameter. In addition, positivity for the neural crest cell marker CD271 reached a maximum of 92.2%, and was 90.4% in the strain evaluated on day 7.
The cells used were human iPSC strains 1231A3 and 201B7. The culture period was 9 days for strain 1231A3 and 7 days for strain 201B7, and CD271 positivity was measured by flow cytometry.
Detailed validation data for this method have been published in an application note. Future development of the CELLFLOAT series is also expected to include application to other cell types and validation of scale-up conditions.
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